| 质粒名称: | pF151 pcDNA3.1(+)SOD1WT |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 26397 |
| 存储实验室: | Elizabeth Fisher |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 (+) |
| 载体出品公司: | Invitrogen |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 5400 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | SOD1 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 470 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | EcoRI(未破坏) |
| 3' 克隆位点: | XhoI(未破坏) |
| 5' 测序引物 | CMV-F |
| 3' 测序引物 | BGH-rev |
|
文献引用方法 |
|
| 材料和方法部分: | pF151 pcDNA3.1(+)SOD1WT was a gift from Elizabeth Fisher (Addgene plasmid # 26397 ; http://n2t.net/addgene:26397 ; RRID:Addgene_26397) |
| 参考文献部分: | Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM. PLoS One. 2010 . 5(3):e9541. 10.1371/journal.pone.0009541 PubMed 20221404 |
买家导航
载体基本信息
载体图谱质粒图谱和多克隆位点信息
SOD1WT质粒图谱.png)
载体简介
载体序列
LOCUS pF151 pcDNA3.1(+)SOD1WT 5872 bp ds-DNA circular SYN 06-AUG-2018 DEFINITION synthetic circular DNA ACCESSION . VERSION . KEYWORDS pF151 pcDNA3.1(+)SOD1WT SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 5872) AUTHORS Stevens JC, Chia R, Hendriks WT, Bros-Facer V, van Minnen J, Martin JE, Jackson GS, Greensmith L, Schiavo G, Fisher EM TITLE Modification of superoxide dismutase 1 (SOD1) properties by a GFP tag--implications for research into amyotrophic lateral sclerosis (ALS). JOURNAL PLoS One. 2010 . 5(3):e9541. PUBMED 20221404 REFERENCE 2 (bases 1 to 5872) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58 http://www.snapgene.com FEATURES Location/Qualifiers source 1..5872 /organism="synthetic DNA construct" /mol_type="other DNA" enhancer 14..393 /label=CMV enhancer /note="human cytomegalovirus immediate early enhancer" promoter 394..597 /label=CMV promoter /note="human cytomegalovirus (CMV) immediate early promoter" primer_bind 548..568 /label=CMV-F /note="Human CMV immediate early promoter, forward primer" primer_bind 642..661 /label=T7 /note="T7 promoter, forward primer" promoter 642..660 /label=T7 promoter /note="promoter for bacteriophage T7 RNA polymerase" primer_bind complement(1242..1259) /label=BGH-rev /note="Bovine growth hormone terminator, reverse primer. Also called BGH reverse" polyA_signal 1248..1472 /label=bGH poly(A) signal /note="bovine growth hormone polyadenylation signal" rep_origin 1518..1946 /direction=RIGHT /label=f1 ori /note="f1 bacteriophage origin of replication; arrow indicates direction of (+) strand synthesis" primer_bind complement(1605..1624) /label=F1ori-R /note="F1 origin, reverse primer" primer_bind 1815..1836 /label=F1ori-F /note="F1 origin, forward primer" primer_bind complement(1955..1975) /label=pBABE 3' /note="SV40 enhancer, reverse primer for pBABE vectors" promoter 1960..2289 /label=SV40 promoter /note="SV40 enhancer and early promoter" rep_origin 2140..2275 /label=SV40 ori /note="SV40 origin of replication" primer_bind 2202..2221 /label=SV40pro-F /note="SV40 promoter/origin, forward primer" CDS 2356..3150 /codon_start=1 /gene="aph(3')-II (or nptII)" /product="aminoglycoside phosphotransferase from Tn5" /label=NeoR/KanR /note="confers resistance to neomycin, kanamycin, and G418 (Geneticin(R))" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" primer_bind complement(2410..2429) /label=Neo-R /note="Neomycin resistance gene, reverse primer" primer_bind 3020..3039 /label=Neo-F /note="Neomycin resistance gene, forward primer" polyA_signal 3324..3445 /label=SV40 poly(A) signal /note="SV40 polyadenylation signal" primer_bind complement(3361..3380) /label=SV40pA-R /note="SV40 polyA, reverse primer" primer_bind 3415..3434 /label=EBV-rev /note="SV40 polyA terminator, reverse primer" primer_bind complement(3494..3510) /label=M13 rev /note="common sequencing primer, one of multiple similar variants" primer_bind complement(3494..3510) /label=M13 Reverse /note="In lacZ gene. Also called M13-rev" primer_bind complement(3507..3529) /label=M13/pUC Reverse /note="In lacZ gene" protein_bind 3518..3534 /label=lac operator /bound_moiety="lac repressor encoded by lacI" /note="The lac repressor binds to the lac operator to inhibit transcription in E. coli. This inhibition can be relieved by adding lactose or isopropyl-beta-D-thiogalactopyranoside (IPTG)." promoter complement(3542..3572) /label=lac promoter /note="promoter for the E. coli lac operon" protein_bind 3587..3608 /label=CAP binding site /bound_moiety="E. coli catabolite activator protein" /note="CAP binding activates transcription in the presence of cAMP." primer_bind complement(3725..3742) /label=L4440 /note="L4440 vector, forward primer" rep_origin complement(3896..4484) /direction=LEFT /label=ori /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of replication" primer_bind complement(3976..3995) /label=pBR322ori-F /note="pBR322 origin, forward primer" CDS complement(4655..5515) /codon_start=1 /gene="bla" /product="beta-lactamase" /label=AmpR /note="confers resistance to ampicillin, carbenicillin, and related antibiotics" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS LIKHW" primer_bind 5278..5297 /label=Amp-R /note="Ampicillin resistance gene, reverse primer" promoter complement(5516..5620) /gene="bla" /label=AmpR promoter primer_bind complement(5695..5714) /label=pRS-marker /note="pRS vectors, use to sequence yeast selectable marker" ORIGIN 1 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca 61 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct 121 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta 181 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac 241 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt 301 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag 361 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat 421 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat 481 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc 541 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc 601 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg 661 gagacccaag ctggctagcg tttaaactta agcttggtac cgagctcgga tccactagtc 721 cagtgtggtg gaattccatg gcgacgaagg ccgtgtgcgt gctgaagggc gacggcccag 781 tgcagggcat catcaatttc gagcagaagg aaagtaatgg accagtgaag gtgtggggaa 841 gcattaaagg actgactgaa ggcctgcatg gattccatgt tcatgagttt ggagataata 901 cagcaggctg taccagtgca ggtcctcact ttaatcctct atccagaaaa cacggtgggc 961 caaaggatga agagaggcat gttggagact tgggcaatgt gactgctgac aaagatggtg 1021 tggccgatgt gtctattgaa gattctgtga tctcactctc aggagaccat tgcatcattg 1081 gccgcacact ggtggtccat gaaaaagcag atgacttggg caaaggtgga aatgaagaaa 1141 gtacaaagac aggaaacgct ggaagtcgtt tggcttgtgg tgtaattggg atcgcccaat 1201 aacgctcgag tctagagggc ccgtttaaac ccgctgatca gcctcgactg tgccttctag 1261 ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac 1321 tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga gtaggtgtca 1381 ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg aagacaatag 1441 caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa ccagctgggg 1501 ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt 1561 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt 1621 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc 1681 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga 1741 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc 1801 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt 1861 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct 1921 gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt agggtgtgga 1981 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca 2041 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc 2101 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc 2161 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag 2221 gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc 2281 ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca agagacagga 2341 tgaggatcgt ttcgcatgat tgaacaagat ggattgcacg caggttctcc ggccgcttgg 2401 gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc tgatgccgcc 2461 gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga cctgtccggt 2521 gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac gacgggcgtt 2581 ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct gctattgggc 2641 gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa agtatccatc 2701 atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc attcgaccac 2761 caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct tgtcgatcag 2821 gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc caggctcaag 2881 gcgcgcatgc ccgacggcga ggatctcgtc gtgacccatg gcgatgcctg cttgccgaat 2941 atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct gggtgtggcg 3001 gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct tggcggcgaa 3061 tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca gcgcatcgcc 3121 ttctatcgcc ttcttgacga gttcttctga gcgggactct ggggttcgaa atgaccgacc 3181 aagcgacgcc caacctgcca tcacgagatt tcgattccac cgccgccttc tatgaaaggt 3241 tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc ggggatctca 3301 tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt tacaaataaa 3361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt 3421 tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct agctagagct 3481 tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc acaattccac 3541 acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga gtgagctaac 3601 tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg tcgtgccagc 3661 tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg cgctcttccg 3721 cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc 3781 actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga aagaacatgt 3841 gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc 3901 ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa 3961 acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc gtgcgctctc 4021 ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg ggaagcgtgg 4081 cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc 4141 tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc ggtaactatc 4201 gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc actggtaaca 4261 ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg tggcctaact 4321 acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca gttaccttcg 4381 gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc ggtggttttt 4441 ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat cctttgatct 4501 tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt ttggtcatga 4561 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa 4621 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac 4681 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga 4741 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgagacc 4801 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca 4861 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta 4921 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg 4981 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc 5041 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg 5101 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt 5161 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt 5221 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata 5281 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc 5341 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac 5401 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa 5461 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct 5521 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat 5581 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc 5641 cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc tcagtacaat 5701 ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt tggaggtcgc 5761 tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg acaattgcat 5821 gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg cc //
